G2 phase chromatin lacks determinants of replication timing
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چکیده
منابع مشابه
G2 phase chromatin lacks determinants of replication timing
DNA replication in all eukaryotes follows a defined replication timing program, the molecular mechanism of which remains elusive. Using a Xenopus laevis egg extract replication system, we previously demonstrated that replication timing is established during early G1 phase of the cell cycle (timing decision point [TDP]), which is coincident with the repositioning and anchorage of chromatin in th...
متن کاملChromatin Determinants of the Eukaryotic DNA Replication Program
0715) Chromatin Determinants of the Eukaryotic DNA Replication Program by Matthew L. Eaton Department of Computational Biology and Bioinformatics Duke University
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The major DNA methyltransferase, Dnmt1, associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand. In view of the slow kinetics of Dnmt1 in vitro versus the fast progression of the replication fork, we have tested whether Dnmt1 associates with chromatin beyond S phase. Using time-lapse microscopy of mammalian cells expressing green-fluo...
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Mammalian genomes are partitioned into domains that replicate in a defined temporal order. These domains can replicate at similar times in all cell types (constitutive) or at cell type-specific times (developmental). Genome-wide chromatin conformation capture (Hi-C) has revealed sub-megabase topologically associating domains (TADs), which are the structural counterparts of replication domains. ...
متن کاملChromatin and DNA replication.
The size of a eukaryotic genome presents a unique challenge to the cell: package and organize the DNA to fit within the confines of the nucleus while at the same time ensuring sufficient dynamics to allow access to specific sequences and features such as genes and regulatory elements. This is achieved via the dynamic nucleoprotein organization of eukaryotic DNA into chromatin. The basic unit of...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 2010
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb.201002002